Tuesday, March 21, 2017

Amnion processing

Our next week in the lab was spent processing Lil's amnion. This was a tedious process as it involved stretching and cleaning the amnion. Normally, this would not take very long, however, the collected amnion was quite dirty so we had to spend extra time cleaning it and making sure there were no external particles lodged in it. The amniotic sac itself is pretty big as it has to contain the entire foal. This means we have to stretch out the amnion and cut out smaller portion to use in the product. This is further complicated because some parts of the amnion have holes, which we have to cut around. Once we do that we set out the amnion to dry overnight. That is as far as we have gotten with processing the amnion. Thanks for reading!

Dr. Barcelo showing us how to clean the amnion.



Diagram showing where the amnion is in respect to the fetus.

7 comments:

  1. Hey Nikash--I met with Brandon, and I hear you are getting pretty good with a shovel! I also hear you have been on a few overnights. Be careful with the mini horses coming in--they can be stubborn.

    Your comment on amnion processing was interesting. Is the product manufacturing what you expected/anticipated?

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    1. Hello Ms. Gathas,
      Coming into the lab I actually was not sure what to expect when manufacturing the product. I have enjoyed my time so far, and Dr. Barcelo has been there every step of the way to explain everything we do. However, we still have not completed all the steps of manufacturing any of the product yet (it is a pretty tedious task that requires days of waiting for certain steps), so I will update you on that as soon as we do it.
      About the mini horses, we spent all of this past Saturday putting up boards in the stables so the mini-horses can't crawl through the railings. I will post pics of the finished stable soon.
      Thank you for your comment!

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  2. Hey Nikash!

    You're awesome for being so methodical about the amnion :).

    What are your thoughts on being able to work with other seniors on an SRP? Looking at others' SRPs, would you suggest a partnership for our rising seniors?

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    1. Hey Anna!
      Having other people I know in the lab with me definitely made my transition from school to the lab a lot more smoother. Oftentimes if we have questions we can just work it out together if our lab mentor is unavailable. Other than that it just makes my time in the lab more enjoyable, since I get to spend it with my friends. I would definitely suggest having a partnership for our rising seniors' SRPs.
      Thank you for your comment!

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  3. Nice one, Nikash! Could you explain why you use the amnion versus other parts around the embryo (eg. chorion)?

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  4. Hey Jaeyoung!
    We avoid using other parts around the embryo (such as the placenta and chorion) because these parts are in contact with the mother's blood. Our lab mentor explained to us that certain organisms may have an allergic reaction to the mother's blood so it is best to only use the amnion, which is separated from the blood by the chorion. Additionally the amniotic membrane is rich in stem cells, which helps make regenerative medicine possible.
    Thanks for the comment!

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  5. This is impressive Nikash! Looks like you have gathered a lot of information and are enjoying working here. This project seems to have some challenging work hours but it is a very unique project experience for a high school student. Look forward to discuss more about your work when we meet on Monday.

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